The RNFL and overall retinal thickness closely match those of the tomograph the SRF is much smaller than in the tomograph because of dehydration during histologic processing. ( B) Stevenol’s blue–stained plastic section. The color scale spans 4 × 10 −10 (black) to 10 −6 (white) of the incident power. Interpolation berween pixels was performed to improve image readability. The sampled pixel size is 3.8 (vertical) by 20 (horizontal) µm. The nasal retina appears on the far right. Posterior Optical Coherence Tomography detects very subtle morphological changes in ocular structures and is therefore essential in diagnosing and monitoring many retinal diseases, such as AMD, macular oedema (one of the complications of diabetic retinopathy or vascular occlusions) and macular epiretinal membrane. Blood vessels (BV) in the optic disk appear as characteristic dark spots.
The RNFL thickness varies between 70 and 90 µm, increasing toward the optic disk. Identifiable structures are, from top to bottom, vitreous, retina (RNFL, red subjacent retina, yellow to light blue), subretinai fluid (SRF), retinal pigment epithelium (RPE), and choroid and sclera. The retina temporal to the disk is on the left. The tomographic image corresponds to a section of the retina and optic disk along the papillomacular axis. It is used in research, medical diagnostics, guided surgery, industrial processing, and non-destructive testing. ( A) Cornea and lens were removed before OCT scanning and the OCT beam was delivered through the vitreous medium and focused on the retina. Optical coherence tomography is a powerful technique for non-destructive 3D imaging of tissue and materials. OCT has been widely used in numerous clinical applications and is becoming popular as a promising technology in dentistry. Eye bank specimens were kept at 4☌ and measured within 24 hours after death. Optical coherence tomography (OCT) is a noninvasive diagnostic technique providing cross-sectional images of biologic structures based on the differences in tissue optical properties. Optical coherence tomograph of human retina and optic disk in vitro (A) and histologic section of the same specimen (B).
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